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Whooping cough in South-East Romania: a 1-year study

joi, 20 iulie 2017
 

Abstract

The incidence of whooping cough in Romania is substantially underestimated, and, as noted by the health
authorities, this is mostly due to the lack of both awareness and biological diagnosis. We conducted a 1-year
study in Bucharest in order to assess the circulation of Bordetella pertussis, the main etiological agent of
whooping cough. Fifty-one subjects suspected of whooping cough were enrolled. Culture, real-time PCR, and
enzyme-linked immunosorbent assay were used for laboratory diagnosis. Whooping cough patients (63%)
were distributed among all age groups, and most were unvaccinated, incompletely vaccinated, or had been
vaccinated more than 5 years previously. Bordetella holmesii DNA was detected in 22% of the bordetellosis
cases; these patients included adults; teenagers; and, surprisingly, young children. B. pertussis isolates were
similar to the clinical isolates currently circulating elsewhere in Europe. One isolate does not express
pertactin, an antigen included in some acellular pertussis vaccines.

Sorin Dinu a, Sophie Guillot b,c, Cristiana Cerasella Dragomirescu d,e,  Delphine Brun b,c, Ştefan Lazăr f,
Geta Vancea f, Biatrice Mariana Ionescu g, Mariana Felicia Gherman g, Andreea-Florina-Dana Bjerkestrand g,
Vasilica Ungureanu d, Nicole Guiso b,c, Maria Damian a
a- Molecular Epidemiology Laboratory, “Cantacuzino” National Institute of Research-Development for Microbiology and Immunology, 103 Splaiul Independenţei, 050096 Bucharest, Romania
b- Institut Pasteur, Molecular Prevention and Therapy of Human Diseases, 25-28 rue du Dr Roux, 75015 Paris, France
c- URA-CNRS3012, 25-28 rue du Dr Roux, 75015 Paris, France
d- Respiratory Bacterial Infections Laboratory, “Cantacuzino” National Institute of Research-Development for Microbiology and Immunology, 103 Splaiul Independenţei,
050096 Bucharest, Romania
e- “Carol Davila” University of Medicine and Pharmacy, Department of Microbiology, 103 Splaiul Independenţei, 050096 Bucharest, Romania
f- “Dr Victor Babeş” Clinical Hospital for Infectious and Tropical Disease